Secondary myofibers appear at around 50 60 days based on the template of the primary myofiber surface.
The PCR reaction contained 1X Perkin-Elmer (Norwalk, CN) PCR buffer, 3 mM MgCl2, 200 µM (each) deoxynucleoside triphosphate, 200 nM of the forward and reverse primers, 5 units of Taq polymerase, and 0.5 2 µL of DNA template (for primary PCR) or 2 µL of primary PCR product (for secondary PCR) in a total 100-µL reaction mixture.
As the template caused the formation of primary macropores the activation created a secondary system of micropores which resulted in a significant increase of the specific surface area up to about 1400 m2 g−1.
The digested DNA was then ligated with a DNA adaptor (Clontech) and the resulting DNA fragments were used as templates for two rounds of primary and nested PCR amplifications using two adaptor-specific primers together with two myog specific primers (GSPs) in the first exon (GenBank accession number EU934044).
The sense of "information" as used in the Central Dogma of molecular biology, which states that information cannot flow from protein to DNA, is more like a fit of template, or the primary structure of the protein sequence compared to the sequence of the DNA base pairs.
We believe that because the template is first amplified in a course of primary PCR, mismatches between primary PCR fragments and secondary PCR primers is more forgiving and allows for productive amplification.
Also included were 5 pmol of wild-type forward primer (5′-GCTCTGATAGGAAAATGAGATC-3′) and reverse primer (5′-GTGGAAAAATAGCCTCAATTC-3′), with 2 μl of template from the primary PCR.
This pilot study explored the challenges of implementing a computerized template in primary care to facilitate communication on TCAM for paediatric eczema.
Only the template/pseudoknot (t/PK) domain of hTR is illustrated; the template region primary sequence is shown maximally base-paired to the template affinity oligonucleotide.
Molecular models of homodimeric SP-B, based on templating the primary sequence of SP-B onto the known 3D-structures of NK-lysin, suggested that SP-B may assume the closed and/or opened saposin conformations when interacting with lipid monolayers or bilayers [4], [5], [28], [29].
