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Automatic calibration is achieved by attaching a magnetic template of known area.
Phenoloxidase and GFP primers were validated by running serial dilutions with a template of known quality and the efficiency of the reactions were determined to be greater than 98%%.
A negative PCR control without template and a positive PCR control (GAPDH) with a template of known amplification cycle were included.
Restrictions such as limited template of known proteins, low quantity, and lack of sensitivity of equipment are all impediments that should be overcome before definitive studies can be accomplished [ 15].
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We quantified the viral RNA using a standard curve generated using S-segment templates of known copy number.
Sequence alignments between these proteins and templates of known structures are obtained from a PSI-BLAST sequence search [ 66].
Fold recognition involves profile-profile alignment of the query sequence to a template database of known folds.
The first step is the identification of a template protein of known structure.
The printed dye microarray is a semi-stable, scalable template of mixtures of known composition for testing algorithm accuracy and to some extent, instrumental limitations.
BCCP domain of ACCA3 (Rv3285) protein of Mtb was modeled by MODELLER 9v6 [36] based on its homology with multiple templates (Figure 9) of known NMR or crystal structures of BCCP or BCCP containing proteins (E. coli BCCP 1bdo, Propionibacterium shermanii transcarboxylase:1dcz, Bacillus subtilis BCCP 1z7t, P. horikoshii BCCP 2d5d and Human acetyl Co-A carboxylase:2ejm).
To obtain control cDNA template of a known quantity for generation of standard curves, product from PCR reactions was isolated from agarose gels with the CONCERT™ Rapid Gel Extraction kit (Marligen).
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