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Here, α represents the density of pores, which is close to 1 × 1010 cm−2 in the present experiment according to the scanning electron microscope image of the AAO template (not shown here); k is the dielectric constant of Al2O3 (k = 7.6), and tBE and tTE correspond to the thicknesses of the bottom AZO layer and the top AZO layer, respectively, i.e., 12 nm.
Consistent with this fact, lowering the concentration of Asn improved the incorporation efficiency of Asp analog C6 in this template (not shown).
Further control reaction mixtures, either without template (not shown) or RT enzyme (not shown), were uniformly negative.
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Electrostatic surface mapping of the ASP-2A model shows that it, in common with other aspartic proteases including both templates (not shown) is strongly negatively charged, on both faces of the molecule but in particular at the substrate binding cleft.
In collaboration with the authors, 3 6 representatives from the medical secretaries and the nurses identified work tasks for the questionnaire each in two 2-hour group sessions, using recently developed detailed work-flow charts as templates (not shown).
These liver mRNAs were clearly detectable by RT-PCR when cDNA from whole larvae was used as template (data not shown).
No incorporation as well was observed with double-stranded (DS) plasmid DNA used as template (data not shown) which has already been reported for Xenopus LSE [35].
We confirmed the human specificity of our primers by lack of an amplicon using cDNA from healthy non-manipulated mouse liver as template (data not shown).
In order to function well in samples with low bacterial/host cell ratios, primers were selected not to match the human genome, and tested not to render PCR amplification with human DNA as template (data not shown).
Primers pairs gave no signal in PCRs lacking template (data not shown).
Similar results were obtained with the Ebf3 template (data not shown).
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