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The experimental results show that the surface-imprinted material MIP-PEI/SiO2 has excellent combining affinity and recognition selectivity for the template molecules of pirimicarb.
We suggest, therefore, that on the basis of our proposed criteria, the moa microsatellite dataset is of high fidelity despite representing template molecules of c. 600 to 5000 years of age [10].
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Amino groups were used for immobilization of the template molecules on the magnetic surface and additionally to react with the terminal vinyl groups of cross-linker, ethylene glycol dimethacrylate (EGDMA), by the Michael addition reaction.
31P MAS NMR spectra of the as-synthesized samples showed unusual peak intensities suggesting an effect of the template molecules on the phosphorus resonance.
This approach is fully effective only when there are amplifiable template molecules for each of the two alleles of each marker.
The time-resolved fluorescence decay analysis was used to investigate the specificity and affinity of the binding of template molecules to the MIP.
It is obvious that the binding amount of template molecules to MIP nanoparticles were much higher than that of NIPs.
A further element of reliability in our work is that the starting copy number of template molecules for ancient or modern DNA preparations is similar and also the type of DNA is the same (human mtDNA).
Sequencing a mixture of 100 template molecules, one of which is mutant, in a single tube would yield an electropherogram in which the dominant signal at the mutated position would be the wild-type base.
The amount of initial template molecules, number of assays used, primer concentration, annealing time and annealing temperature are key parameters that influence the sensitivity, specificity, efficiency and reproducibility of targeted preamplification.
Leakage of the residual template molecules is one of the biggest challenges for application of molecularly imprinted polymer (MIP) in solid-phase extraction (SPE).
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