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Due to the covalent attachment of a PCR primer on polyacrylamide matrix and the retarding effect of the polyacrylamide hydrogel matrix itself, as the polony reaction proceeds, the PCR products diffused radially near individual template molecule to form a bacteria colony-like spots of DNA molecules.
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It is obvious that the binding amount of template molecules to MIP nanoparticles were much higher than that of NIPs.
The time-resolved fluorescence decay analysis was used to investigate the specificity and affinity of the binding of template molecules to the MIP.
The seeded supports were calcined and treated in a synthesis solution free from organic template molecules to form ZSM-5 films on the supports.
The most significant release is achieved with the template molecule, corresponding to 30% reduction in the binding capacity.
DNA segments complementary to the ends of the template molecule were designed to reach Tm values of ≥ 53°C.
Control wells containing no template have been used to exclude the presence of contaminating template molecules and to identify potential primer-dimer products from the dissociation curve analysis.
The PurAmp method presented in this study is performed in a single tube from cell lysis to cDNA or genomic DNA amplification, thus eliminating possible loss of template molecules due to procedures such as phase separation and recovery, repeated washing and re-suspension of nucleic acid pellets, elution from binding matrices and vessel-to-vessel transfer.
Indeed, a number of telomerase RNA template molecules appear to be a limiting factor of telomere elongation in budding yeast.
At heterozygous positions sequenced bidirectionally, the minimum number of original template molecules required to produce a signal is only four: forward reference, forward alternate, reverse reference, and reverse alternate.
This may result from the fact that the qPCR measurement is dependent on quantification of the RNA standards by UV absorbance; it is known that UV absorbance measurements can overestimate the number of amplifiable template molecules due to the presence of contaminants, damaged RNA, or off-target oligomers.
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