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Yeast two-hybrid constructs for BRP were obtained by PCR using the corresponding cDNA as template (modified from Wagh et al., 2006).
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The RFP template for vectors V1662 to V1664 was modified from the Clontech dsRED2 construct using site-directed mutagenesis to incorporate 73 single point mutations (resulting in 36 different amino acids) as outlined [ 19, 18].
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Food intake assay was modified from [53].
The test was modified from [24].
Note: modified from [ 5].
Table modified from [52].
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