Finally, two main threats to the validity of this study are: i) potential bias in the papers' selection, as this was performed by one reviewer, who is the primary author; and ii) some relevant use case templates may not be included.
However, as 3DPCR amplification occurs very close to the denaturation temperature, some templates may not be fully denatured, which would block polymerase elongation, thus rendering amplification less efficient.
In particular, a model based on a single template would be highly similar to the chosen template and may not be the best representation of the target structure.
These templates, however, may not be an appropriate approach for directing landscape-level forest management strategies.
The use of universal primers on DNA extracted from samples with a dominant DNA template (i.e. diet samples) results in primarily the dominant template being amplified, which may not be of interest [6], [21], [22].
The challenge of locating a homologous template for HR repair may not be an obstacle in the compact genome of O. dioica, where up to several hundred copies of each locus [ 25] are available for recombination in endocycling cells.
The algorithms in [22] used a number of future frames to generate pattern templates, and thus, it may not be suitable for some applications where frame delay cannot be tolerated, such as real-time interactive communication.
A limitation of the template technique is that it may not be applicable in patients with a bilateral fracture or patients with a history of a distal radial fracture or deformity on the other side.
It should be noted that the use of template matching to detect brain potentials may not be novel and has been addressed before.
President Obama recognizes that our form of government and our way of life should be an inspiration but may not be the template for every other country in the world.
