Sentence examples for template isolated from from inspiring English sources

Restriction endonuclease DpnI targets methylated DNA sequences and thus can cleave the DNA template isolated from most E. coli strains but not the PCR product.

In a preliminary study, we performed similar PCR using a cDNA template isolated from a hepatoma tissue of a patient with autoimmune hepatitis.

Briefly, two cDNA samples synthesized from same RNA template isolated from zebra mussel feet were labelled with ALEXA 555 and 647 respectively.

Long-range PCR was performed with the primers 1 F-5′-GGAACTAACCAAACGGAGCA-3′ and 1R-5′-AGGATTGCTTGAGCCTGAAA-3′ usingenomicic DNA template isolated from patient samples showing a large deletion in exons 11 13.

Plasmid is pure double-stranded DNA of fixed length, whereas the reverse transcriptase-PCR template isolated from tissues is single-stranded cDNA or a mRNA cDNA hybrid with a target-specific secondary structure.

Primary PCR amplification reactions were performed in a 25  μl reaction using ReddyMix (ABgene, Epson, UK) containing 5 pmol of forward (5′-TCATAATGCTTGCTCTGATAGGA-3′) and reverse (5′-GGCCAAAAATTTAATCAGTGGA-3′) primer and 5  μl of gDNA template isolated from tumour cells.

Fig. 4 LAMP assay using 20 different DNA templates isolated from the same Neisseria gonorrhoeae standard strain (ATCC 49926), but from different colonies, at a concentration of 1 pg/µL.

The LAMP experiment was then repeated using 20 different DNA templates isolated from the same N. gonorrhoeae standard strain (ATCC 49926), but each from a different colony, to further test for sensitivity and replicability (as described above).

To further confirm the reliability of LAMP, the experiment was repeated using 20 different DNA templates isolated from the same N. gonorrhoeae standard strain (ATCC 49926), but each from a different colony, at a concentration of 1 pg/µL.

We were able to detect PCR products on DNA templates isolated from normal cells but not from the MCF10A series of cell lines (data not shown).

Twenty-three of these apoptosis-related genes were cloned and sequenced using cDNA templates isolated from BmE-SWU1 cells.