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The original Guard template is separated into C4 and D4 constrained templates and a new Guard template.
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In preliminary syntheses, the circularized products and unreacted linear templates were separated on a 1.5 mm DPAGE and visualized by UV shadowing, excised from the gel and electroeluted.
The annealed primer-template was separated from unincorporated radiolabeled TTP using a Micro Bio-Spin 30 column (Bio-Rad), which was pre-equilibrated with 20 mM Tris, pH 8.0, 100 mM KCl.
Twenty-four generatemplatesates were separated on 1.5% agarose gel, and the band of the correct size was gel purified.
Uncleaved transcription products from the 61 nt-long pGEM-T easy upstream sequence templates were separated in and eluted from polyacrylamide gels.
The primer-annealed DNA templates were separated from excess labeled primer or remaining template DNA on 8 % non-denaturing polyacrylamide gel followed by gel-purification and used in a primer extension assay by Taq DNA polymerase.
Accordingly, the PSS-ANP template did not influence the electrical characteristics of the GaN-based LED because the active area of the GaN-based LED with the PSS-ANP template was separate from the optical reflective area.
In semiconservative DNA replication an existing DNA molecule is separated into two template strands.
Template candidates were separated into clusters using an affinity propagation algorithm (Frey and Dueck, 2007).
Template-bound proteins were separated by 15%% SDS-PAGE and analyzed by Western blot (Fig. 4a, b) or Coomassie blue staining (Fig. 4c).
All PCR reactions were carried out using 5 ng of template DNA; digestion products were separated on either 2% (rs1079597, rs1800498 and rs1800497) or 2.5% (rs1799732) agarose gels and visualized using ethidium bromide and UV illumination.
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