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In contrast, in running start assays with Dpo4 where a stretch of a template is replicated in the presence of all four dNTPs starting several positions upstream from a DNA lesion, a significant accumulation of intermediate products corresponding to incorporations opposite and adjacent to the lesion has been observed for a number of DNA lesions.
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In this way, the micro- to nano-sized patterns of the original master Si template were replicated on various substrates, including a flat Si substrate and curved acryl substrate, with high fidelity using UV nanoimprint lithography.
In the presence of the target DNA, the template DNA is replicated via RCA and the products bridge the gap between two electrodes.
It has been suggested that the discontinuous replication of the lagging strand by the Okazaki fragments increases the frequency in which the template of the lagging strand is in single-strand (ss) DNA conformation relative to the template of the leading strand, which is replicated continuously (Frank and Lobry 1999).
In conjunction with an earlier experimental result, an explicit model for a template dehydropolymerization process has been developed that describes how lignin primary structure is replicated.
Once viral proteins are synthesized, the viral RNA is replicated, beginning with negative-strand RNA synthesis using the genomic RNA as a template.
On the other hand, if the RNA base is positioned opposite the mutation site, the mismatched template should be replicated faithfully.
The MSD template could be replicated in other contexts to facilitate multi-constituency, multi-sector collaboration for long-term engagement and to develop effective implementation agendas with shared ownership.
Ban said he hoped the Kenyan model could act as a template that could be replicated across the continent.
In this paper, however, although we describe the main inputs and activities, we seek not as much to provide a specific template that can be replicated, but rather to highlight the fact that such services can be provided in rural settings - and thus, not require of pastoralists that they relocate to urban settings to receive adequate care (Table 1).
"Our desire is to create a sustainable social model template that can be replicated and adapted to meet the emotional and spiritual needs of different socio-economic populations in various geographic areas.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com