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Homology based modelling could be performed only on 11 malarial phosphatases, with conserved domain region present in the models (Data not shown) while the remaining 7 do not contain conserved domain region in the homology based models, especially in the cases where query sequence similarity with the template is poor.
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Further experiments show that the partial load rate is located in 0.79%–1.81% when the parallelism of template (mold) is good, and the partial load rate of tie bars between 8.59%–11.46% when the parallelism of template (mold) is poor.
However, the stability of this method is poor, and the template manufacturing and stamping process still need improvement.
But Wuppertal is poor.
This is poor here.
There is poor care, sometimes very poor.
PfuUltra II Fusion was not used for clinical samples because its amplification efficiency on variable virus templates is often poor [13].
This is in agreement with the observation that sequences containing LNA are poor templates for most DNA polymerases [ 16].
While the 3D structure builder produces reasonable conformations for molecules without rings or with ring systems for which a template exists, the results may be poor for molecules with more complex ring systems or organometallic species.
Moreover, correlation between QFI-PCR and Qubit by template 'functionality' for these samples was poor (R = 0.30).
The template is there.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com