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Although the yield of template is low, this step allows for screening large numbers of proteins.
The homology-based methods can have a high accuracy of predictions when close templates are identified, but the accuracy decreases sharply when the sequence identity of target and template is low (e.g. <30%).
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Conformation Z-scores of both the model and the template were low, though the model's side chain planarity and inter atomic distances were good.
However, nanotubes still exist despite of the increased deposition time, because Ni2+ions concentration in the margin region of the templates is low and can not be supplemented from the whole solution in time.
Karrer et al. described the Monte Carlo effect using plant material suggesting that the PCR reproducibility could be limited when the number of available templates is low.
Yet such proof-reading polymerases could introduce biases when the quantity of mutant molecules in the template DNA is low.
In some cases, however, the template number was low or template recovery poor such that only presence or absence of a variant could be called.
In addition, it is important that you address the following comments in order to improve the paper: Specific comments: 1) It is clear from Figure 1B that the major limitation for low-frequency SNPs is low template abundance, rather than sequencing errors.
In practice, however, this cost is low because templates are checked for matching before attempting allocation and usually they contain just a few placeholders.
In some cases, however, the template number was low or recovery poor such that no more than presence absence of a variant could be called.
When the queried template is present in low copy numbers in both the test and reference sample, the variation in ratio between the two templates is dependent on the stochastic distribution of both transcripts, resulting in a multiplication of stochastic effects.
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