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The schematic illustration for preparation of Co-NC catalyst via using of Co-MMT flat template is indicated in Fig. 1.
The calculated new bond position for the substrate is indicated with a dotted line, and the guide atom of the template is indicated by an asterisk.
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(a) Scheme of the nanostructured Si template (the Si is indicated in blue and Au in orange) and (b) the relative SEM image in cross-view.
On top of each template, primer extension direction is indicated by → whereas × marks a non-extended scenario.
A denied connection occurrence is indicated by one of the three template parameters received by CheckErrorOnConnection template structure, i.e., the first one that has a boolean type.
b Induced nucleotide mismatches to generate mismatch "bubbles" in 1 × 1, 2 × 1, and 2 × 2 oligonucleotides that differ from the template sequence are indicated by being underlined.
Non-template bases are indicated in lower case.
Template positions corresponding to pause sites are indicated.
A template was used to indicate the surface area to inoculate.
Presence of a predicted product from a deletion template was taken to indicate that the wild-type gene was still present in the genome and presumably functional.
The surface sites were sampled as described by Alfa et al [ 22] by using a pre-moistened swab that was rubbed vigorously over the inoculated 1 cm2 surface area (a template was used to indicate the surface area to sample) and then placed into 2 mLs of sterile reverse osmosis (RO) water.
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