If an exogenous repair template is also supplied, the induced double-strand break can be repaired by homology-directed repair and sequence alterations encoded in the repair template are incorporated into the genome [27].
An internal control (primer pair and template) was incorporated into every reaction mixture, including negative controls.
Approximately 50 ng of genomic DNA or 100 ng of cDNA template were incorporated in each reaction.
In viruses, genetic exchange takes the form of recombination [ 4], when information from two template strands is incorporated to a daughter strand, or reassortment [ 5], in which viruses with segmented genomes exchange chromosomes.
However, in the former case, if primer cleavage and extension do occur on a mismatched template, the alternate allele is incorporated into the extension product and a new amplicon is created which is now a perfect match to the primer.
The overhang sequence is incorporated into template DNA ends in the early rounds of PCR.
In this process, the cement powder is incorporated into a liquid foam, which acts as a template for macroporosity.
The 5'-tail region is non-complementary to the DNA template and increases the melting temperature of the primer once the tail is incorporated into the PCR product.
With indirect labeling, aminoallyl-dUTP is incorporated into control and tester DNA with PCR of 3 cycles after the reassociated DNA templates are amplified.
