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The level of gene repair obtained with IDLVs carrying both ZFNs and the donor template is higher than previously reported repair rates obtained with the same pair of ZFNs under similar conditions using an analogous reporter system (Urnov et al., 2005).
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In addition, if the probability of the chromosome becoming a template is high (P CRTT = 0.9), but the probability of the ribozymes becoming a template is not low enough, the spread of the chromosome is not favored.
Interestingly, ATPase activity in the presence of supercoiled template was higher than that observed for DnaA box alone.
These results on the standard deviations of the location errors mean that the precision of the proposed framework using the BSE-filtered templates was highest among the three templates.
As shown in Fig. 1, the average number of words used by the students to record their experiments is higher in the Template condition compared to the No Template condition consistent with the expectation that the Template condition would lead to the capture of more information.
Thus, the dNTPs Km on duplex DNA template is 2 10 times higher than on ssDNA template.
The gray value of the LAMP product from 3 copies of initial DNA template was much higher than that of the cutoff line.
The principle of FP derives from the fact that the degree of polarization of a fluorophore (in this case FAM) is inversely related to its molecular rotation as indicated in Scheme 2. As shown in Fig. 4, at low concentrations of nanoparticles (0.78 and 1.56 μg/mL), FP of Myr-NQA-MINs after incubation with FAM labeled template was significantly higher than FP of NQA-MINs and NINs.
The chromosome (as a circular RNA) may become a template with P CRTT (the probability of a circular RNA turning to a template), which should be higher than P LRTT (the probability of a linear RNA turning to a template), with which individual ribozymes (as linear RNAs) may become templates.
The rationale behind VORESCORE is the following: if the prediction method mistakenly ranks a certain template best, e.g. because of chance similarities with the wrong and missing sequence similarities with the correct template, chances are high that the VORPSI rescoring will also be mistaken.
However, we quantified the number of templates and the lowest number of templates was 2,300, resulting in a theoretical depth of 0.04% (1/2300), but for most samples the template input number was higher.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com