DNA sequencing performed for 15 samples with missense mutation detected P53 gene mutation only in six samples, in another six cases, the mutated template was difficult to detect, in the final two cases the DNA analyses did not revealed a P53 gene alteration (for one sample DNA was not analyzed).
They feel that generic business plan templates are difficult to match to very specific real-world businesses.
It is attributed that the periodic nano-sized pores of AAO templates were difficult to be filled with PC at too low process temperature.
The inner diameters of the PANI nanotubes by the "soft" templates were difficult to control and sometimes the nanofibers or nanoparticles were by-produced [10, 11].
Performance decrease is caused by the fact that transformed biometric templates are difficult to align (like in BCSs) in order to perform a proper comparison and, in addition, information is reduced.
And because each resource belongs to only one stack in Heat specification, a template extraction is difficult for shared resources such as logical routers which connect VPN or the Internet not to belong multiple stacks.
Based on this data, we concluded that the presence of 25% of mutated template can lead to misinterpretation of sequencing results and may suggest that 25% of mutated template could be difficult to detect during sequencing of DNA.
When sequence identity is below 30%, it is difficult to recognize the best template and generate accurate sequence-template alignments, so the resultant models have a wide range of accuracies (Chakravarty et al., 2008; Sanchez et al., 2000).
It is difficult to use the template-matching method to identify adherent cells with high yield.
