The results, shown in Figure 2, indicate that the reaction using FPE RNA as a template was consistent with that seen for the high quality commercial RNA.
The higher incidence of PCR recombination in the amplicon templates is consistent with previous findings of increased rates of PCR recombination with increased concentration of template DNA [42], [43].
Allowing newly synthesized positive-sense genomes to remain in the complex and act immediately as replication templates is consistent with previous reports indicating a coupling between translation and replication as we still require initial, infecting genomes to be translated before transitioning to replication (Novak and Kirkegaard, 1994).
Since there were a number of templates with similar E-values and since the homology was remote, an evaluation of the models themselves was carried out using the statistical potential DFIRE [43] and the top four were structurally aligned to each other to determine whether the models based on different templates were consistent with each other.
Meanwhile, if the number of domains in the DCS prediction is >3 but less than that in the first cluster, we substitute the DCS prediction with the domain information of the first cluster when the domain boundaries in >50% of templates are consistent (i.e. differences are ±20 residues).
In each case UvrD1 647 was able to unwind the substrates, although a slightly higher concentration of UvrD1 647 than wild-type UvrD was required to unwind the 3′ tailed template, which is consistent with the reduced affinity of the truncated protein for single-stranded DNA that was observed during the ATPase assays.
Penalty terms for warping distance insure that loci in an individual subject's brain are never warped more than 3 cm to locations in the template brain, a distance that is consistent with reports on the variability of the locations of functional loci (e.g., Watson et al. 1993; Dougherty et al. 2003).
Pol β can bypass abasic sites and 8-oxo-deoxyguanine in short DNA gaps by utilizing the downstream templating base for coding and is consistent with its low deletion frameshift fidelity in gapped DNA.
The window size is consistent with the template size in GTM, i.e., each window has a size of 75 × 75.
As shown in Table 1, the observed masses for single glyceronucleotide or deoxyribonucleotide incorporation on Template A are consistent with the calculated values.
