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The growth of Co nanowires in the InP membrane template is carried out in an electrochemical cell with a three-electrode configuration: a Pt counter electrode and a Pt pseudo-reference electrode, which are both immersed in the Co electrolyte.
The second PCR using the amplicons from each sample as template is carried out by adding 5 µl of purified product, 1 µl (10 µM) of each of the forward and reverse barcoding primers, 8 µl of the 5-Prime HotMasterMix 2.5×, and 5 µl of UltraPure Distilled Water (Invitrogen).
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Gene correction with single-stranded oligodeoxynucleotide (ssODN) as repair template was carried out as previously described with some modifications (Peters et al., 2013).
A study of SAPO-11 synthesis using typical compositions of starting gel with low times of crystallization and gels with low proportion of template was carried out.
The removal of the PS template was carried out, and a porous Ag film, with regularly distributed nanopores (Figure 3c), was available for chemical etching to obtain the SiNW arrays.
Reverse transcription of each DNase-treated RNA template was carried out with random primer and M-MLV Reverse Transcriptase (Promega).
Additionally, semi-nested PCR reactions using 20F/1492R and 20F/1406R amplifications as template were carried out similar to bacterial 16S rDNA reactions, with 30 cycles instead of 35.
The PCR using genomic DNA of transgenic Arabidopsis leaves as template was carried out with the vector specific primers: 1304-F (5'GandCTTCCTCTATATAAG3') and 1304-R (5'GGACAACTCCATGAAAAG3').
An inverse PCR with the plasmid pSasC4074 as a template was carried out for the construction of subclone 1 and subclone 2 by using the primers CHsasCSub1rev/CHsasCSub1for and CHsasCSub2rev/CHsasCSub2for, respectively.
Registration of each participant's functional T2*-weighted to high resolution T1-weighted scans and subsequently to the MNI52 standard brain template was carried out using FLIRT, with 7, and 12 degree-of-freedom linear transforms, respectively [54], [57].
A negative control without DNA template was carried out in each run.
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