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In eukaryotes, the transcription of a template is affected by its chromatin state, which can be regulated by post-translational modifications of terminal tails that protrude from the histone core.
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The final outcome of such research is affected by many factors, the choice of template being a crucial one.
The nonspecific RNA-synthesis assay used in the robotic approach does not identify which step in the cycle is stimulated by HN and HC proline substitutions or inhibited by other alterations; even template engagement could be affected, as faster recycling of RNA polymerase could also increase RNA yield.
The level of order of the cubic template is also found to be affected by the addition of the particles.
Kinetic analysis revealed that both template/primer binding and dNTP incorporation are affected by the Trp229Tyr mutation.
On a template for school assemblies the charity calls it "a day of reflection, allowing people to remember or think about all those people who are affected by wars, both in the past and now".
were affected.
DNA synthesis along the ssDNA template was not critically affected when saturating amounts of NCp7 (1 NCperper 5 nucleotides) were added with increasing amounts of RT (from 6 nM to 500 nM), as shown after an electrophoresis of the deproteinized DNA products (Figure 3A).
The mismatches between the primers and the DNA template are likely to affect the efficiency of PCR amplification, thus giving rise to weak signals or false negative results, as has been demonstrated in studies involving the use of primers MGS-2 and MgPa-903 [27] [28], [28].
A number of factors affect whether readthrough occurs when an mRNA template is being translated.
The template is there.
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