Suggestions(1)
Exact(3)
The similarity between the amplification profiles for both the primer:template interaction and the runway sequences of these two DNA polymerases is remarkable.
In order to analyze the data relevant to the random region, we filtered the data based on a 4 bp window (runway), 6 bp window (primer:template interaction), 8 bp window (primer:template interaction and 2 bps of runway), and 10 bp window (primer:template interaction and 4 bps of runway) as demonstrated in Figure 2.
The bias profiles for the two different families of polymerases have an R of 0.15 (n = 4,096, p = 0.0000) for the primer:template interaction and an R of 0.49 (n = 4,096, p = 0.0000) for the runway sequences.
Similar(57)
This study describes the design and synthesis of molecularly imprinted polymers (MIPs) as patterned thin films, based on molecular modelling of functional monomer-template interactions and validation by NMR-spectroscopy.
Positive amplification bias towards increasing GC content was observed for the six base pairs of primer:template interaction for all of the commercial DNA polymerases tested, and several preferentially amplified sequence motifs were identified.
Overall, QTT-A and PGT-A DNA polymerases have a similar amplification profile with an R of 0.98 (n = 4,096; p = 0.0000) for the primer:template interaction despite the fact that they were ordered from two separate vendors and likely work in different buffer conditions.
Therefore, the specific location of the GC content contributes to the amplification bias throughout the primer:template interaction.
There is positive amplification bias towards increasing GC content for the six base pairs of primer:template interaction for all of the commercial DNA polymerases tested.
The runway sequences in the described experiments did not have a clearly defined trend with regards to GC content like the primer:template interaction.
It is reasonable to consider that a primer:template interaction that has a tendency to be of the A form or a primer:template interaction that can be more easily conformed to the A form is preferentially bound by the DNA polymerase, ultimately resulting in biased amplification.
When the OBV for each runway motif is plotted with the sequences arranged in order of increasing GC content, the bias profile does not demonstrate a similar pattern to the primer:template interaction.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com