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Hierarchical clustering based on the top 200 differentially expressed genes from the 10 μg reference data again showed clear separation of the two cell lines for all template inputs, with strong correlation seen between the 10 μg reference differentially expressed data and the 1 ng data (Pearson correlation 0.89), and between the 10 μg reference and the single cell data (Pearson correlation 0.78).

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As expected, the correct signal rose sharply in proportion to template input with thermal cycling (Figure 3C, blue trace).

The "drift" in GeneChip performance and RNA targets being reliably targeted with lowering RNA template inputs can be seen by Principle Components Analysis (Figure 4 C).

FFPE tumor DNA presents a dual challenge for mutation testing, namely requirements for low template input quantities combined with template damage from the fixation and embedding process that resist amplification by PCR.

}. }; Now the CJPEGDec artifact template can be presented (see Listing Listing 15 CJPEGDec is the template artifact related to JPEG Decoder concept) and should be later instantiated with two template input parameters given by the data types of SamplingType and Color Space features.

The median template input per fragment is 120 with an interquartile range of 50-500.

Beads for the fragment library were amplified with a two copy per bead template input, and the paired-end libraries were amplified with a one copy per bead input.

Conserved positions are typically monomorphic even in deeply sequenced samples with high RNA template input.

However, the results obtained with this test also depend on template input and setting the reading threshold for obtaining Ct values, two parameters needing further standardization.

In some cases, template input limits accuracy more drastically.

For sequencing from DNA, 80 ng were used as template input.

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