Sentence examples for template input for from inspiring English sources
DOI: http://dx.doi.org/10.7554/eLife.11282.027 In addition to quantification of template input for each sample, we separately estimated the recovery of sequence variation in each of the six sequenced amplicons.
Country X template inputs for response include a functional, dedicated command and control center with room and information and communications technology equipment and services to accommodate up to 40 personnel during an event (Technical Appendix Table 3).
For such samples, the same dCt values were yielded for a broad range of template input, from 150 ng/reaction down to 1.5 ng/reaction (Figure 3A).
Our results demonstrated an insensitivity to template input amounts for this particular method, thus validating the results of the experiments carried out on various cells and tissues.
To demonstrate an insensitivity to template input amounts for this particular method, we ascertained the reliability of our test over a wide range of mtDNA copies, from 10 to 10. Results are displayed in Supp. Figure S1.
GAPDH was used as a reference gene to correct for template input.
FFPE tumor DNA presents a dual challenge for mutation testing, namely requirements for low template input quantities combined with template damage from the fixation and embedding process that resist amplification by PCR.
However, the results obtained with this test also depend on template input and setting the reading threshold for obtaining Ct values, two parameters needing further standardization.
We applied the TransCount model as an approach to eliminate unreliable data based on quantitative measures of template input in the initial amplification and for the threshold determination.
However, we quantified the number of templates and the lowest number of templates was 2,300, resulting in a theoretical depth of 0.04% (1/2300), but for most samples the template input number was higher.
From this initial generation of parameter sets, a set of input files for the simulation engine was generated from the template input files.
