Exact(4)
Figure 5(c) shows the alignment between the final model and the chosen template, indicating that the predicted tertiary structure was preserved during the MD simulation.
Note that the RNA DNA hybrid on the antiHIV-1 template is not stronger than that on the HIV-1 template, indicating that the pause is not determined by a weak hybrid as was proposed earlier (Palangat and Landick, 2001).
As shown in Figure 7, no products were amplified using either spheroid body or R. gibba DNA as template, indicating that the identified (pseudo genes do not have functional counter parts encoded elsewhere in the endosymbiont's genome.
Our structural model for glp-4 VARS-2 has a backbone RMSD of 0.8 angstroms (determined by pyMol alignment) from the starting template, indicating that the crystal structure can be used for homology modeling of the C. elegans protein with only modest backbone rearrangement.
Similar(56)
A single ~1.5 kb PCR amplification product was obtained using either genomic DNA or cDNA of common bean as template, indicating that this gene contains no introns.
Second, no F1 probe binding was observed in the absence of the target sequence in the transcribed segment of the template, indicating that hybridization was specific for the F1 target.
14.0 million raw bases were obtained in 55,206 reads with 99.02% of the reads mapping back to the template, indicating that almost 30 Mbp can be obtained from a library of 131,000 molecules prepared from 500 pg input material, in this case at a specific yield of 220,824 454 FLX reads (254 bp reads) per nanogram input DNA.
On the other hand, a ∼0.5Kb fragment was amplified when cDNA was used as template, indicating that an intron of ∼0.9Kb in size is present in the 5'-UTR sequence of the atf1+ transcript.
In vitro synthesis and labeling experiments using the three main STOX1 (A, B, C) isoform-coding plasmids as templates indicated that the bands detected by the antibody in placenta and JEG-3 cell extracts correspond to these three isoforms.
This observation with the different sized templates indicates that the effect of GTP concentration on the activity of the enzyme is consistent, regardless of the sequence and size of the template.
On the other hand, no change in PCR marker between the two templates indicates that the amplified locations are protected from the digestion by DNA methylation.
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Justyna Jupowicz-Kozak
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