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On the other hand, a ∼0.5Kb fragment was amplified when cDNA was used as template, indicating that an intron of ∼0.9Kb in size is present in the 5'-UTR sequence of the atf1+ transcript.
Figure 5(c) shows the alignment between the final model and the chosen template, indicating that the predicted tertiary structure was preserved during the MD simulation.
A single ~1.5 kb PCR amplification product was obtained using either genomic DNA or cDNA of common bean as template, indicating that this gene contains no introns.
Note that the RNA DNA hybrid on the antiHIV-1 template is not stronger than that on the HIV-1 template, indicating that the pause is not determined by a weak hybrid as was proposed earlier (Palangat and Landick, 2001).
Second, no F1 probe binding was observed in the absence of the target sequence in the transcribed segment of the template, indicating that hybridization was specific for the F1 target.
As shown in Figure 7, no products were amplified using either spheroid body or R. gibba DNA as template, indicating that the identified (pseudo genes do not have functional counter parts encoded elsewhere in the endosymbiont's genome.
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A series of dilutions of a typical template indicated that we could detect expression over a broad range.
The template indicates that more resources are required in the delivery of health interventions via ACCHSs, due to their comprehensive nature.
The improved rate and timeliness of documentation with the new note template indicate that a short template with increased opportunity for free text may be more efficient and effective for capturing high-level or collaborative work.
Quantitative real-time PCR amplification with bacterial and archaeal primers using the same DNA template indicated that considerably less of the DNA present in the sample was derived from archaea than from bacteria.
A structural modeling of GmF3G2″Gt using the 3D structure of a grape flavonoid 3- O-glucosyltransferase, VvGT1, as a template indicated that three substituted amino acids (V142, S149, T183 in GmF3G2″Gt-b) were located far from the enzyme active sites.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com