Sentence examples for template in vitro from inspiring English sources
Interestingly, whereas Rad51/Rad54 together gave efficient D-loop formation on chromatin template in vitro, this activity was abolished if Rad51 was replaced by RecA [6].
We conclude that both types of trans-splicing seen at a single locus can be readily reproduced on a purified template in vitro using reverse transcriptase.
Affinity-purified, epitope-tagged RDR6 was capable of synthesizing long complementary RNA strands using a single-stranded RNA (ssRNA) template in vitro [39].
First we evaluated the effects of RanBP7 and histone H1 on a simple chromatin template in vitro, in extraction buffer (100 mM KCl, 1 mM MgCl2, 0.1 mM CaCl2, 10 mM K-HEPES pH 7.7, 50 mM sucrose) supplemented with ATP but in the absence of cytoplasm.
Their biochemistry nicely shows that APRIL/pp32 exclusively stimulate vRNA synthesis from a cRNA template in vitro.
In all, 10 mg of RNA was used to synthesize cDNA, which was used as a template in vitro transcription according to the manufacturer's instruction.
In this study we evaluated novel computationally designed surface selective laser sintering (SSLS) scaffolds for their biocompatibility as templates, in vitro and in vivo, for human fetal femur-derived cell viability, growth and osteogenesis.
The data indicate that SIL was able to prevent NS5B binding to RNA templates in vitro.
Importantly, Cr VI) treatment of DNA templates in vitro induces DNA polymerase arrest with the most potent arresting lesions at G runs [24], [25].
When pKS-Luc, pTM1-Luc or pTM1-2A plasmids were used as templates, in vitro polyadenylation was performed with poly(A) polymerase (Invitrogen).
The term 'prion-like' is used because there is no evidence that α-synuclein aggregates are transmitted between individuals (that is, there is no evidence for infectability), but as reviewed below, α-synuclein aggregation can clearly seed and self-template in vitro and in cultured cells under conditions that favor the entry of preformed amyloid intocells.
