Sentence examples for template in three from inspiring English sources

This mixture was used as template, in three 96-well 50 μl PCR reactions using primers complementary to the adapters, and the ~100-bp products were PAGE purified.

The same RNA from each of ten life stages that was used for pooling was now used separately as a template in three replicate transcription reactions, performed with the QuantiTect Reverse Transcription Kit (Qiagen, Germantown, MD, USA) following the manufacturer's instructions.

"Great Scott" departs from the standard American-opera template in one significant way: this is not an adaptation of a well-known novel, play, or film but an original story.

Such versatile porous support catalyst played as a template in two levels.

First, the platform implemented the game template in two environments such as the Nokia series 60 platform and the Symbian OS.

This template, in one way or another, has guided hack reviewers as well as influential critics like Otis Ferguson, James Agee, Pauline Kael and Andrew Sarris.

5' flap sequences (e.g., a 54-mer oligonucleotide rich in G and C bases) attached to the 5' end of a primer is highly efficient during linear PCR (by using a single primer to amplify DNA template in one direction, because there is no partner primer, the amplification is linear rather than exponential).

Serially diluted DNA for each pathogen was used as a template in four triplicate calibration curves.

One DNA sample was used as template in six PCR reactions with each of the six primer pairs to compare the relative amount of PCR product.

Sequence analysis of individual repair events revealed that the yellow gene was replaced by attP from the ssODN template in five of eight clones.

Genomic DNA was extracted from a single individual and used as template in ten independent PCRs in order to amplify a specific part of the Ig domain.