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This may have led to the assignment of the mass loss at 300°C to 420°C to the PSt template in several reports [28 30].
Presence of genomic DNA in the cDNA template was ruled out by using mRNA as template in several independent Real Time PCR and end point PCR reactions.
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The automatically generated templates in several runs with parameter adjustment were carefully considered to represent a single form, distinct from other signals.
Peak measurements from experimental samples were compared to a standard curve generated by mixing in vitro transcribed RNA from BYDV-PAV and PAS templates in several ratios (ranging from 1 9 to 9 1 PAV PAS) prior to RT-PCR amplification and sequencing.
Brain-specific and ovary-specific A-to-I RNA editing sites were further verified by comparing cDNA sequences with their corresponding genomic templates in several cell lines from brain, colon, breast, bone marrow, lymph, liver, ovary and kidney tissue.
The blast search against the protein databases with psTic62 [Swiss-Prot: Q8SKU2] as a template resulted in several sequences from which just two correspond to the full-length form of the mature psTic62: one from Arabidopsis thaliana [GenBank: NP_188519] and another from Oryza sativa [GenBank: ABG65881].
The sensitivity and specificity of these assays was higher than individual assays based on direct antigen detection and standard PCR against a range of control templates and in several hundred clinical specimens.
To synthesize Pt nanoparticle without aggregation, site-specific synthetic methods in the template were attempted in several research groups [7, 8].
Experiments show that the proposed method significantly outperforms linear classifiers as a template matching method in several image classification tasks, and is much more computationally efficient than other commonly used non-linear classifiers.
Although not included in the formal templates, in 2010 several informants reported they had incorporated EIDM in their performance objectives and they would be using their annual reviews to demonstrate how they are developing and applying EIDM knowledge and skills.
The primer annealing temperature determines that the primer completely binds to the template or partially mismatches the template in one or several bases.
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