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Jaimie Ngyuen, a junior at Wesleyan University, which uses the Student Aid Services template, in real life received about $1,500 more in aid than the calculator indicated.
Serial dilutions were generated and 5 µl aliquots were included as template in Real Time PCR analyses for quantification of genome copy numbers (see below).
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cDNA samples were further diluted 50 times in nuclease-free water before use as template in real-time PCR experiments.
A 6 µL digested aliquot was then used as a template in real-time PCR (final 20 µL volume).
The reverse-transcribed cDNA of each reaction was diluted 5-fold with sterile water and used as template in real-time quantitative PCR reactions.
cDNA was used as template in real-time PCR assays based on SYBR Green detection with the ABI Prism 7000 Sequence Detection system.
The cDNA was used as the template in real-time quantitative PCR; the qRT-PCR conditions and analysis were similar to those used in the study of Gao and associates [30].
Myocyte total RNA was isolated using the RNEasy Mini Kit (QIAgen), and cDNA to be used as template in real-time PCR was produced using the First-Strand Synthesis Kit (Invitrogen).
Subsequently, 2 μL of cDNA was used as the template in real-time quantitative PCR reaction.
All cDNA samples were 1 5 diluted with RNase-free water for use as template in real-time PCR.
The cDNA products were then diluted 50-fold with deionized water before use as a template in real-time PCR.
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