Bands where it was unclear whether they were the result of extensible or non-extensible dimers, including artefact bands that were extremely faint in both template and template-free lanes, appeared in only the template-free but not the template lane, or were very small but dark when compared to amplicon bands, were excluded from analysis.
In the embryo in which correction using the repair template could be detected, the template sequence was found in only a subset of cells, and the remaining cells in this embryo had probably been corrected using the maternal version of the gene.
Contigs from different chromosomes will inherit template strands independently, and by chance, the templates will be the same in only half of all libraries.
The template strands used in these experiments differ in only one position, the templating base, 3′ to the 2-AP probe.
Actually, MLR-CI achieves better AUCs in only three templates, and better sensitivities in only four templates.
As expected, efficient amplification of DNA template in whole blood, occurred only in samples supplemented with both 1,2-propanediol and trehalose.
In the models, the secondary structure is similar to that of the template; variations only occur in loops, as often for modeled structures.
The telomerase RNA template sequence (CCCCCCAUU) has been detected in only these two hypothetical non-coding RNA genes among all the predicted genes in the present 100% complete nuclear genome.
The first — the so-called "event-based model" — regards informed consent as a passive signature of a standard unreadable template, performed only once in each medical pathway.
Notably, the remaining SMARCAL1 residues, 7 13 and 28 32, were disordered in this model because the algorithm does not generate structural models for residues lacking the template and only 17 residues in the UNG2 peptide are ordered.
