The surface and cross-section morphology of the PANI/PC composite film was characterized by environmental scanning electron microscopy, indicating that the honeycomb ordered PC film plays the role as template in controlling the morphology of the PANI/PC composite film.
PCR-grade water was distributed as template in negative control reactions.
The IL (which was composed of TPA-C and TPA-B) was then evaluated as a soft template in order to control the Ag nanostructures.
This could be due to an external contamination of the water used in place of template in the negative control.
Occasionally fluorescence was detected in no- template controls in later cyles (>35 cycles), and melting curve and gel analysis indicated the source was primer dimers.
Next we determined systematically the dosage effect of Cas9 RNPs and HDR templates on HDR efficiency in control and nocodazole synchronized cells.
All centres included at least 2% of the samples in duplicate, no template controls in every plate, and a random mixture of affected and unaffected carriers.
The role of the polymeric surfactant as a soft template is key in controlling the morphology and the properties of the obtained PANI dispersions.
The standard curves were generated for each gene using serial dilutions of positive control template in order to establish PCR efficiencies.
Genomic DNA was used as a control template in parallel PCR reactions to confirm the expected electrophoretic pattern of the retroposed sequences.
