Sentence examples for template in combination from inspiring English sources

3 µl of the cDNA template in combination with 0.2 mM dNTP mix, 2 mM MgSO4, 0.25 µM each primer, and 1 U Platinum Taq high fidelity polymerase (Invitrogen) was used in a standard PCR reaction.

To generate the template for 5'Nuc-specific dsRNA (length: 496 bp) production by in vitro transcription (IT), a plasmid containing the full length 5'Nuc coding sequence (pET17b:5'Nuc) was used as PCR template in combination with following primers: 5'Nuc IT) sense (5'- TandACGACTCACTATAGGGGCAGACAGCTTGTACGACCA-3') and 5'Nuc IT) antisense (5'-TAATACGACTCACTATAGGGTCATGAATTCGATCACGGAA-3').

The new template in combination with the refined machine-learned score function was used to identify amino acid substitutions that either destabilized interactions with d-Thy or resulted in structural changes favoring bound l-Thy in the phosphoryl-acceptor site.

Each of the CPDL variants was constructed by overlap extension PCR using AtFatB-par as template in combination with the primers listed in Table 1 then cloned into the pBC expression plasmid.

This study uses emulsion templating in combination with microstereolithography to fabricate PolyHIPE scaffolds with a tightly controlled and reproducible architecture.

The use of analogs of 2-ME as templates, in combination with a chromatographic separation of the analytes in the sample, overcame the problem of the template bleeding.

The use of templates in combination with user defined variables with the functionality of the template system was new to the current EHR products used in the Stockholm region.

This method is adapted from Wellenreuther et al. [ 18] who combined template switching in combination with agarose gel mediated size fractionation prior to full cDNA PCR amplification to isolate long full-length genes using microgram amounts of purified human poly-A mRNA.

Non-PCR based template amplification in combination with ligation of growing DNA strands reduces the rate of spontaneous errors.

The approach of the template system in combination with various local EHR systems can facilitate the sharing and reuse of validated clinical information from different health care units.

It was concluded that the pJANUS™-02-001 performsperforms adequately for use as a calibrator template DNA in combination with the GTS-40-3-2 GTS-40-3-2 GTS-40-3-2 GTS-40-3-21].