During DNA replication, ssDNA is present at the lagging strand template but also on the leading strand template in cases of transient uncoupling of the MCM helicase from the polymerase (Pacek & Walter, 2004).
Whereas the length of coiled coil was much longer than the template in cases like myosin II and V, models were generated by a protocol that involves repeating the same template over and over again with an overlapping region spanning 70 residues (Margaret et al, oral communication; December, 2010).
Genomic DNA was used as template and in case of cas1 (because of its internal NcoI restriction site) with plasmid pGFP-CAS1 as template.
The connections are repeated with the mirror image of the template structure, in case it is not symmetrical.
Thus, hemicatenanes or related topological structures may facilitate homology-mediated annealing to the same template strand in case of direct repeats.
All IDs contain a reference to the ORF models that were named according to the template used: in case of cDNA or EST clones according to the clone name and in case of primary cDNA according to the ORF prediction.
When the CM-based ranking is used to rank the RF templates in cases where the highest predicted TM-distance is >0.5 (19 cases in the RA benchmark) the model accuracy improves in 10 cases, leading to the selection of a significantly better model in 5 cases (PDB ID: 1BQL, 1IQD, 1FBI and 1HZH, 4.3, 3.8, 7.7, 5.0 and 3.7 Å RMSD to 2.1, 2.1, 3.7 and 2.4 Å RMSD, respectively).
The proposed image matching uses phase scrambling in order to protect the original information of templates visually in case there is leakage of the template.
DSA is the use of a patterned template, in this case a nanoimprint mold, to guide the self-assembly of BCPs into nanopatterns with good long range order and precise size and location which can then be used to fabricate a higher quality nanoimprint mold.
