The template used for modeling was identified via the template identification tool (with default parameters), which performs a primary sequence comparison of the query sequence with those in a structural database.
The heavy and light chains templates were identified using the SWISS-MODEL workspace template identification portal.
More specifically, both used a two-track approach for template identification and ranking.
As a result, MESSA limits tools for structural template identification to those few that are known to perform best.
The template used in this modelling was selected from template identification function in the SWISS-MODEL program.
The results obtained by previous methods were compared with the results obtained by Swiss-model server for template identification (http://swissmodel.expasy.org).org
The BLAST E-value limit was set at 1.0e-6 while template identification searches were performed, selecting for the best template through the SWISS-MODEL workspace [ 44, 45].
This is especially important when there are no structural homologs existing in the database or the template identification techniques cannot find good templates (Zhang, 2008b).
