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The optimum reference template given a symbol candidate can be obtained using the variational technique (see [17]) (11).
We tested the ability of our procedure to recover the structure of a sub-cavity template given a set of noisy instances of a template structure.
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Indeed, the first refinement pass using the 4D template gives a significant improvement for the Foreman and Soccer video sequences.
Figure 4b shows the cross-sectional image of Co-Ni binary nanowires embedded in the alumina template giving a bright contrast as marked by arrows.
With regrowth of GaN on nanopores, the threading dislocation density has been reduced to 1.2 × 108 cm−2 The surface of the 2.0 μm thick n-GaN on nano-ELO GaN template gives a RMS roughness of 0.32 nm when compared to 0.29 nm for conventional GaN template.
An angular-angular correlation between the measured map and the template gives a score of the correspondence between the two that is rotation independent, and the absolute value of this quantity is also independent of clockwise and counter-clockwise orientation around the supra-barrel center.
The RT-PCR reaction for E. kanangrensis-cGMP PDE using either eye or brain cDNA as a template gave a product in both cases (Additional file 3E-F).
The PCR results show that, using wild-type DNA as a PCR template gave a band of the size expected in the case of no T-DNA insertion.
The reaction conditions used in both rounds of the nested PCR consisted of the following: 20.5 μL 2× PCR Master Mix (1.5 mM MgCl2) (Thermo Scientific), 900 nM of each primer and 1.5 μL DNA template, giving a total of 25 μL per sample.
PCR using primers to cover the whole ASDE sequence with DNA extract templates gave a PCR amplicon of approximately 4 kb only.
All minor modifications of the original, approved QC-method must be re-validated according to a template given in the original submission and a statistical equivalence should be shown between the original and modified QC-methods.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com