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A small aliquot (~2 μl) of the resultant PCR product was then used as template for single-strand DNA (ssDNA) generation using only the N (nested) primers for all tags.

Fig. 5 Hot-spot generation using a colloidal crystal template.

Template generation is implemented using a MySQL database to store semantic relationships between standard experiment types from the MGED Ontology (Whetzel et al., 2006) and the sample annotation and experimental variables expected for each experiment type.

The recombinant plasmid was used as template for in vitro transposon mutagenesis using Template Generation System II kit (TGS, F-702; Finnzyme, Finland).

The database and template generation web forms can be used via ArrayExpress (at http://www.ebi.ac.uk/cgi-bin/microarray/magetab.cgi), or installed and used locally.

PCR was used for template generation with the GC-rich PCR System Kit (cat. number: 12 140 306 001), Roche Applied Science; 34 cycles of annealing at 50°C were performed.

In brief, we identified a median-size larva and registered it to every single image stack used for template generation (40 stacks), computed the average transformation and applied it to the median-size larva.

Restriction enzymes used for DG template generation recognize a nested set of 4, 6 and 8 bp restriction sites, providing a flexible depth of analysis and coherent cross-referencing of information derived from different genotyping applications.

RNAi was performed according to published methods using previously reported sequences for template generation [23].

The corrected larva was used as the starting point for the iteratively template generation (Asadulina et al., 2012).

The technique of template-driven dynamic code generation specifically using XML template processing is also general enough to be effective in other domains seeking to solve the issue of portability and abstraction of modeling logic from simulation code.