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Further aging induced the directional assembly of the 2 nm-sized nanoparticles onto the reverse micelle template, generating the nanotubes.
The PCR product was digested with PstI and EcoRI and was cloned between the corresponding sites in the same plasmid that was used as template, generating the plasmid "pET28a PE QQ delta NS5AB-15aa linker-HNP-HIS-KDEL".
The PCR product was digested with AatII and PstI and was cloned between the corresponding sites in the same plasmid that was used as template, generating the plasmid "pET28a PE QQ delta -DTA(1 187)(instead domain III -mutated NS5AB- 15aa lIII -mutatedS-KDEL".
The PCR product was digested with PstI and AatII and was cloned between the corresponding sites in the same plasmid that was used as template, generating the plasmid "pET28a PE QQ delta -DTA(1 187)(instead domain III)- no cleavage site- 15aa linker-HNP-HIS-KDEL ".
The PCR product was digested with BamHI and EcoRI, and was cloned between the corresponding sites in the same plasmid that has been used as template, generating the plasmid "pET28a PE QQ delta RTA-mutated NS5AB-short linker-stalk peptide-HIS-KDEL".
The PCR product was digested with BamHI and XhoI and was cloned between the corresponding sites in the same plasmid that has was as template, generating the plasmid "pET28a PE QQ delta RTA-full 2a JFH1 NS5AB-short linker-stalk peptide-HIS-KDEL".
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Next, applying the problem and question conditions to the problem-text template generates the problem text.
The impregnation of a polymeric scaffold by slurry enabled the manufacturing of a porous network reminiscent of spongy bone (the core) whereas ice templating generated the outer structures (the shell) very similar to compact bone.
PCR3 with primers P1 and P4 on PCR1 and PCR2 templates generates the 5′ integration fragment.
Thus, such molecules would likely be repaired using the intact homolog as the template, generating a 4 0 conversion.
In the absence of Gal4DBD, the nucleosome was moved away from the template end by several hundred base pairs towards the center region of the template, generating a rather broad distribution.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com