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Patient data were entered into the Memopad using a customized template generated with MemoPlus (Hands High Software Inc ,Palo Alto, CA, USA).
In vitro transcripts of preSroC were generated with the T7 Megascript Kit (Ambion) using a DNA template generated with oligonucleotides JVO-8373/JVO-8374 and were hydrolyzed by tobacco acid pyrophosphatase (Epicentre).
The sgRNAs were created by in vitro transcription of a PCR template generated with a unique forward primer containing a T7 polymerase binding site and the gene-specific guide sequence, as well as a common overlapping reverse primer containing the remainder of the sgRNA sequence necessary for incorporation into the Cas9-sgRNA complex.
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Reaction using PCV2 or P1 as template generated a melting curve with a sharp peak, while the melting curve of PRV, PPV or PRRSV was flat.
In [15], decision-directed autocorrelation (DDA) receivers are proposed to detect the current symbol by correlating the current information waveform with a waveform template generated by all previously decoded symbols.
The remaining figures are overlays of axial or sagittal slice of a template brain, generated with the freely available application MRIcroN [78], version beta 2 (http://www.cabiatl.com/mricro/mricron/index.html).html
For normalization, a transformation matrix between the mean image of realigned volumes and the SPM2-EPI (MNI) template was generated with a fourth degree B-spline algorithm and applied to reslice volumes with a voxel size of 3 mm × 3 mm × 3 mm.
DNA templates were generated with the QIAprep 96 Miniprep kit (Qiagen Inc., Mississauga, Canada) and sequenced as described previously [ 56].
The two templates were generated with primers 5'-GCA GTC GAC GGT ACC AAT TCT GAC AGG TGC AGC TTT TCC -3' and 5'-GAT CTT CCT CCT TCC ATA CCT AA-3' (promoter) and 5'-AAA TCT TCG CTA ATC GCA GAG and3' and 5'-CGG TCG AGA CCC GGG GAG CAT AAT CTG GAA CAT CGT ATG GAT AGC CTC CTG ACT CCA GAT TCG GAA C-3' (EDR2 cDNA with HA tag).
The mRNAs were obtained from peripheral white blood cells with the TRIzol reagent (Invitrogen, CA, USA), and template cDNAs were generated with random hexamer primers.
The shape templates generated are matched with shadow silhouettes.
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