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The reviewer also argues that even if the template generated an MCC copy, this reaction may not be functionally relevant because the copy can be generated away from kinetochores.
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As little as 1 ng of C. gigas genomic DNA template generated a visible PCR product.
We found that arranging three copies of the VACV G8R structural model using the human PCNA crystal structure as a template generated a structure similar to the human PCNA trimer (Figures 4 and 5).
Reaction using PCV2 or P1 as template generated a melting curve with a sharp peak, while the melting curve of PRV, PPV or PRRSV was flat.
For a given compound, the example template generates an HTML list of its chemical or other roles.
Elements of combined template are shuffled using shuffle key and hash of the shuffled template generates a unique session key.
Thus, such molecules would likely be repaired using the intact homolog as the template, generating a 4 0 conversion.
The creation of the molecular imprint was favored by the chemical forces of the electroactive template, generating a far higher number of recognition sites than a non-electroactive template.
In the absence of Gal4DBD, the nucleosome was moved away from the template end by several hundred base pairs towards the center region of the template, generating a rather broad distribution.
The sgRNAs were created by in vitro transcription of a PCR template generated with a unique forward primer containing a T7 polymerase binding site and the gene-specific guide sequence, as well as a common overlapping reverse primer containing the remainder of the sgRNA sequence necessary for incorporation into the Cas9-sgRNA complex.
One hundred models of mTRPC3 were made with MODELLER version 9.10 on each of these templates (generating a total of 500 models).
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