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To address this, we developed rPE library amplification, which doubled the number of unique sequence reads per template from two to four in a single PE read.
For three individuals from a single geographic locality (JN), we amplified the same template from two independent PCR to verify strain haplotypes [ 32].
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SWIFT robustness was evaluated by generating a cluster template from one sample, then assigning cells to this template from three experimental replicate samples from the same subject, or from different subjects analyzed under the same conditions on the same day.
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PCR efficiency of each combination of primers was assessed with a control template prepared from two bacterial artificial chromosomes (BACs) encompassing the Kcnq1ot1 region analyzed, as described previously.
Reactions were performed in triplicate using cDNA templates from five tissues samples for each gene.
DNA templates from six specimens of three crustacean species were tested in parallel for the 3 primer pairs employing the same PCR conditions.
Algorithm results were compared against a classification truth based on correlation coefficients between paced QRS complexes and intrinsic rhythm QRS templates from three surface ECG leads.
The set covered 119 bioactive templates from nine categories of ion channel targets and molecular weights of the screening compounds ranged between 150 and 450.
cDNA templates from five normal B cell and ten CLL cells were analyzed for expression of DSP, TRIB2, DUSP1, FOS, JUN, SELPLG, AMICA, MMP9, TYROBP, and LEF1 with taqman probes obtained from Applied Biosystems.
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