Sentence examples for template from each from inspiring English sources

We then extracted a template from each frame of the probe image sequences separately for each quality.

Standard curves covered a range of approximately 8 orders of magnitude and were constructed as previously described [39].The first negative controls contained RNA template from each sample that had not been subjected to RT, and the second controls contained reaction solution without template.

The amount of template from each sample was adjusted until PCR yielded equal intensities of amplification for β-actin.

The 4 internal VPaI-4 primer pairs gave PCR products of the expected size with DNA template from each of 24 of the 41 isolates examined (Table 5).

Total 10 ng of cDNA sample from normal and lesionic areas of thyroid tissue (5 ng template from each area) of each patient was used as the template.

Then, 1 μg of DNA template from each individual was double-digested using the restriction enzymes PstI-HF (20 units/reaction) and MspI (20 units/reaction) in one combined reaction for 3 hr at 37°.

Then, quantitative PCR was carried out using specific primers for exon 10 (see Table S1) Templates from each cell line were prepared in triplicate per target gene as 10 µL reactions (40 ng template, 2X Mesa Green qPCR MasterMix for SYBR assay, 100X forward and reverse primers).

Complimentary DNA (cDNA) templates from each sample were prepared from 1 µg of total RNA primed with oligo dT primers (Pharmacia, Gaithersburg, MD) using 400 units of MMLV reverse transcriptase (Promega, Madison, WI) followed by 30 PCR amplification cycles (94°C for 30 seconds, annealing at 60°C for 60 seconds, and extension at 72°C for 90 seconds).

Therefore, we performed qRTPCR using reverse-transcribed mRNA templates from each tissue.

All sequence data sets were then assembled using templates from each of the 3 BPI3V genotypes.

Reciprocally, templates from each protein in the PDB_SELECT_90 are also searched back against the query structure.