The detection range of the method was assessed on purified DNA templates from different animal origins.
The use of NEMA keys allows templates from different structures to be compared, and allows storage of structures containing a canonical list of templates.
Open image in new window Fig. 1 Agarose gel showing polymerase chain reaction (PCR) results using DNA templates from different governorates with the primer sets LCO1490/HCO2198 for COI.
This observation motivates us to propose a Spectral Feature Alignment (SFA) based method to align workflow DS templates from different domains in a latent space by modeling the correlation between the DI and DS workflow templates in a bipartite graph and using DS features as a bridge for cross-domain Big Data classification.
Thus, it has been suggested that combination of templates from different levels of MNase digestion may alleviate biased sampling of mononucleosome populations [ 58].
Yes, we did real time quantitative RT-PCR using templates from different growth ages and different tissues, and found that CERK1, LYK4 and LYK5 shared a similar expression pattern in all samples tested.
In order to confirm the RNA slot blot, internal primers were designed from each fragment (Additional file 4) and RT-PCR was conducted using cDNA templates from different tissues following standard thermal profiles (Additional file 4).
Thus, 17.5%, 15.4%, and 20.8%, respectively, of reactions would be expected to contain >1 template, of which, given the ratio of populations A and B in each region, 31%, 17.2%, and 28%, respectively, would contain templates from different populations, equivalent to 5.4%, 2.6%, and 5.8%, respectively, of positive reactions, compared with the 4.2% (3/71) observed.
PCR products for MVaf, MVcf and other constructs were prepared using cDNA templates from different sources, such as human brain (MVaf1), HeLa (MVcf), 1205Lu melanoma cells (DLC2), B cells (Bmf), sk-mel melanoma cells (MVaf2 and MVaf3), and clone EST63321 from ATCC (DLC1).
RT-PCR amplification of template RNA from different samples was performed using ExTaq kit (TaKaRa) using manufacturer's protocol.
