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With this knowledge, a template for validation procedures can be build.
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k8101-49685 genomic DNA was used as template for the validation experiment.
One hundred pairs of primers (with PIC values ≥ 0.45 and major allele differences ≥ 3 bp) were selected, and DNA from twenty new maize inbred lines was used as the template for indel polymorphism validation.
Predicted sequence-specific chemical shifts are used as a template for re-assignment and validation of existing resonance assignments.
For validation of these templates, one-step RT-PCR and PCR were performed.
The actual number of viral templates amplified with our protocol was determined for three samples (see Template Number Validation below).
Although synthetic DNA is routinely used in many areas of biomedical research and examples of application of synthetic templates for diagnostic assay validation [12], [23], [24] as well as attempts to create multivalent synthetic test templates [25], [26] can be found in scientific literature, this is the first report of a large scale validation strategy based primarily on synthetic genes.
cDNA samples were used as template for real-time PCR for the validation of miRNA.
1) RT-PCR amplification: For validation of splice variants, nested PCR was performed using 100 ng cDNA templates from the Human Multiple Tissue cDNA Panels I and II (BD Biosciences).
A template validation protocol for TLC and paper chromatography was developed.
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