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In marked contrast to β2-tryptase, the Ser214-Gly219 segment, which normally provides the template for substrate binding, is kinked in α-tryptase, thereby blocking its non-primed subsites.
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On the other hand, the Bam-budding model (Gruss et al., 2013) proposes that the BamA β-barrel uses its exposed edge β-strand, in particular the β1 strand, as a template for the substrate peptide to initiate barrel formation.
The structure of the Slp-76 motif (PDB code 1YWO P) (40) with the sequence PPVPPQRP was next used as a template for our substrate KEEPPSPPQS p P.
Silicon is now widely used for micro/nanoelectromechanical systems (MEMS/NEMS) [2], solar photovoltaic battery [3], template for nanoimprint [4], substrate for quantum dot growth and so on [5, 6].
More generally, the basic model developed may serve as template for other two-substrate two-product reaction mechanisms.
Templates for splicing substrates were generated by digestion of pEZ_ISS/WT with Nar I. Labeled RNA was synthesized from linear plasmid in a 50 µl reaction containing 2 µg of DNA, 0.4 mM each of ATP and GTP, 0.1 mM UTP and CTP, 2.5 µl of [32P]-UTP and [32P]-CTP (ICN), 0.5 mM diguanosine triphosphate and 2 µl of 20 U/µl T7 RNA polymerase Plus (Ambion, Austin, TX).
Synthesized structurally the rigid template having 1,3,5-triazine as core moiety, has been used as template to introduce two appropriately spaced amino groups for substrate binding on the solid surface of silica.
The porous and flexible BC nanofiber is used as a substrate and template for successive polymerization of PPY, which is responsible for such a large areal mass of 13.5 mg cm−2.
To obtain tensile strained silicon layer, germanium is an ideal template substrate for the epitaxial growth, as it has the same crystal structure as silicon but with a larger crystal lattice.
The applications can be extended to the fields such as catalysts, antireflection coatings, template for inorganic growth masks, and substrates for cell culturing providing the patterned surface containing micron-sized features.
Previous works have created a template for the design of new substrate-competitive inhibitors for GSK-3β based on the kinase-specific recognition motif SXXXS p) and on the chemical nature of the regulatory domain of the substrate HSF-1.
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