Sentence examples for template for site from inspiring English sources

The YCA1 open reading frame in the BG1805 plasmid (Open Biosystems, Huntsville, AL). was used as a template for site directed mutagenesis.

pEE6.4/hu3S193 HC was used as a template for site-directed mutagenesis (Gene Tailor™ Mutagenesis SystemenesInvitrogen(Invitroren) or QuickChange II XL Site-Directed Mutagenesis Kit (Stratagene) to introduce the following substitutions in the CH2 and CH3 domains: I253A, H310A, H435A and I253A/H310A [25].

The plasmid containing WT-hSGLT3 cDNA was used as a template for site-directed mutagenesis.

The Tat-FLAG construct served as template for site-directed mutagenesis (Stratagene) to generate the F38A mutation.

Recombinant "wild-type" mAChE was used as a template for site-directed mutagenesis introduced using the QuikChange II Site-Directed Mutagenesis Kit (Stratagene).

The plasmid pHPR [29], which contains HPR cloned downstream of T7 promoter, was used as template for site-directed mutagenesis by overlapping primer extension by polymerase chain reaction.

Human RhoA in pCMV-myc was used as a template for site-directed mutagenesis (QuikChange II Site-Directed Mutagenesis kit; Stratagene), wherein 5 silent DNA basepair mutations were made within the miRNA targeting region of RhoA.

This non-targeted form of RhoA was then used as a template for site-directed mutagenesis in order to generate cysteine to alanine mutants at C16 and C20 (C16/20A myc-RhoA).

To obtain a siRNA-resistant form of BIG1, a HA-tagged version kindly provided by M. Vaughan [55] was used as a template for site-directed mutagenesis using the following sense primer: 5'- GGAACAAAAGATCAGGC(T/A CC(T/A GA(T/C GAATT(T/C GTGGGTTTAGGGC -3' in combination with its antisense primer.

Plasmid pUC Hsp90 then served as template for site-directed mutagenesis (Fig. S6).

The plasmid was also used as a template for site-directed mutagenesis of the WSC1 cytoplasmic tail DNA.