Sentence examples for template for single from inspiring English sources

A total of 1 μg RNA was used as the template for single strand cDNA synthesis utilizing random primers and the Primescript reverse transcriptase (M-MLV, Takara, Japan).

One μg of total RNA was isolated from non-tumor colonic tissues using RNA Bee (Tel-Test, Inc., Friendwood, TX) and used as the template for single strand cDNA synthesis utilizing the Transcriptor First Strand cDNA Synthesis Kit (Roche, Indianapolis, IN) according to the manufacturer's instructions.

From 2 5 micrograms of total RNA, double-stranded cDNA was generated as a template for single-round in vitro transcription with biotin-labeled nucleotides using the Affymetrix cDNA Synthesis and In Vitro Transcription kits (Affymetrix Inc., Santa Clara, CA).

These were used to construct a single-stranded shotgun library that was used as a template for single-molecule PCR.

One microgram of total RNA served as a template for single-stranded cDNA synthesis in a reaction using oligo (dT) primers and SuperScript II (Invitrogen).

A small aliquot (~2 μl) of the resultant PCR product was then used as template for single-strand DNA (ssDNA) generation using only the N (nested) primers for all tags.

For the RT PCR, the total volume (10  μl) of mRNA preheated at 65°C for 5 min served as a template for single-strand cDNA synthesis in a 20  μl reaction mixture containing 3 m M MgCl2, 75 m M KCl, 50 m M Tris-HCl (pH 8.3), 0.5 m M dNTPs, 200  μ M oligo (dT) primer, 20 U of RNase inhibitor, and 200 U of M-MLV reverse transcriptase (Gibco BRL, Gaithersburg, MD, USA) at 37°C for 60 min.

For generating control templates for single nucleotide primer extension (SNuPE) assays and for the cytoplasmic localisation and centrosome amplification assays, a pZeoSV plasmid containing the full-length BRCA1 cDNA with or without UV [ 3] was used.

Current PDB may contain all templates for single-domain proteins according to the seminal studies in Zhang and Skolnick (2005a).

SMRT technology utilizes a sequencing-by-synthesis approach in which a circular DNA molecule is used as a template for a single DNA polymerase.

The amplification protocols utilized here allow buccal cell samples to be used in repeated measures experiments removing the necessity to sample more than once to obtain sufficient template for a single microarray.