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The molecule's shape allows it to unwind and act as a template for replication and transcription.
The template for replication can either be an existing DNA molecule or RNA.
Another possible explanation for the occurrence of VBNC cells after desiccation is that these cells are without functional template for replication of DNA.
The RNP serves as the template for replication that generates viral genomic RNA in the form of an RNP ready to be packaged in the virion.
Consequently, a better approach is to use the silicon stamp as a master template for replication of a daughter, or working stamp based on a cheaper and preferably more flexible material.
P and L complexes have also been suggested as necessary precursors to association with nucleocapsid template for replication [31].
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The double-stranded DNA is unwound and the two single strands form the templates for replication of the chromosome.
We hypothesize that unlinkase activity is utilized by picornaviruses to distinguish templates for translation from those that are to serve as templates for replication or that are encapsidated in virions for further rounds of infection.
VPg is cleaved from picornaviral RNA genomes that are destined to serve as templates for translation; the viral protein is retained on templates for replication as well as genomes that are encapsidated [8], [27], [28], [29], [30], [31].
One potential therapeutic target is the Mcm2-7 (maintenanceome maintenance protein 2 7) eukaryotic replicative helicase, a molecular motor that unwinds duplex DNA to generate ssDNA templates for replication.
The linear telomeric DNA associated with APBs is unlikely to be a major source of templates for replication, however, because it is predominantly short (15) and would not account for the rapid telomere elongation events observed in ALT cells.
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