Sentence examples for template for real from inspiring English sources
100 ng of RNA sample was used as template for real time RT-PCR to determine the expression levels of IFNB1 and DDX58.
Serial dilutions of the cell lysate were generated in a. bid., and 5 µl aliquots were directly used as template for Real Time PCR assays.
RNA was extracted from cells using RNAeasy columns (Qiagen, Valencia, CA, USA), complementary DNA was prepared as recommended Bio-Rad Laboratoriess, Hercules, CA, USA) and used as template for real time PCR.
cDNA was then used directly as a template for real time PCR.
10% of the RT product was used as a template for real time PCR analysis.
The resulting cDNA was used as a template for real time PCR.
Ten ng of cDNA were then used as template for real-time PCR using SYBR Green in ABI prism 7000 (Life Technologies Inc., Burlington, ON, Canada).
The oligonucleotide is detected by the addition of target DNA, designed to hybridise to the oligonucleotide and serve as a template for real-time PCR using the LightCycler.
cDNA served as template for real-time PCR, which was conducted using a QuantiTect SYBR Green PCR kit (Qiagen, UK).
cDNA was used as a template for real-time PCR.
Immuno-precipitated chromatin DNA was then used as a template for real-time quantitative PCR.
