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A comparative model of the target protein is required as input, from which a predicted ligand binding site is automatically extracted and used as a shape and pharmacophoric feature template for rapid screening of large compound collections.
The cDNA was subsequently used as a template for 5' rapid amplification of C-terminal ends (5'-RACE) using 5' universal primers (Clontech), anti-sense TCR beta chain constant region primer, TCRB 5'-ATTCACCCACCAGCTCAGCTCCACG-3' and a high-fidelity polymerase Advantage 2 (Clontech) and incorporating a touch-down polymerase chain reaction (PCR) protocol.
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As one argument goes, this Germany's economy is not a template providing a tangible plan for rapid E.U. growth.
This indicates that, at higher concentrations, the presence of a large number of high-affinity binding sites on the surface of Myr-NQA-MINs offers more opportunity for rapid adsorption of template molecules.
This aspect of Gap5 is still largely unexplored, but we envisage a variety of additional cached tracks for rapid visualization in the template display.
The pHD-DsRed-attP vector was designed for rapid generation of gene-specific donor templates and custom synthesized (DNA2.0; Figure S1).
BLAST searches identified eight novel non-redundant PDI-like consensus sequences, which were used as templates for 5' and 3' RACE (Rapid Amplification of cDNA Ends) extensions using the 5'/3' RACE kit from ROCHE following manufacturer's instructions.
Reverse-transcription of total RNA into the first-strand cDNA was performed by an RT-PreMix kit (SBS Genetech) and a universal dT3AP [oligo dT -containing adaptor primer], which were directly used as templates for RACE (3′ rapid amplification of cDNA ends) with primers MeuICK-F1 and 3AP.
This chiral tetramate scaffold may provide a useful template for fragment-based drug design providing rapid access to novel antibacterial compound libraries.
Similarly, Jiang et al. designed a composite hydrogel scaffold based on PEG and fibrin for vascular tissue regeneration where PEG forms a porous mechanical and structural template for the fibrin polymers to induce rapid vascularization (Jiang et al. 2013).
Plasmid DNA (1 μg) was ethanol precipitated and used as template for in vitro transcription/translation using the Rapid Translation System® (RTS) kit (Roche Applied System).
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