Sentence examples for template for producing from inspiring English sources

The template for producing dsRNA was generated by polymerase chain reaction (PCR) using primers with T7 promoters flanking on the 5′-ends.

For Myc-tagged human cortactin expression constructs, the wild-type human cortactin cDNA subcloned into pcDNA FLAG2AB [43] was used as a template for producing point mutants by site-directed mutagenesis (QuickChange; Stratagene, La Jolla, CA).

RRF-1 amplifies the RNAi response by using the mRNA as a template for producing secondary (2°) siRNAs, which ultimately direct the cleavage of the targeted mRNA by the enzyme, CSR-1 [7].

During gene transcription, the DNA double helix needs to be opened so that one strand can be used as the template for producing mRNA.

Total RNA (200 ng of total RNA) was used as a template for producing double-stranded cDNA and to perform in vitro transcription amplification using the Illumina Total Prep RNA amplification kit (Ambion, TX, USA), following the manufacturer's instructions.

RNA sample (300 ng each) was used as template for producing double-stranded cDNA, and then biotinylated cRNA using the Illumina RNA TotalPrep Amplification Kit (Ambion Inc., Austin, TX, USA).

A new approach, based on a combination of salt and hard templating for producing multi-modal porous carbons is demonstrated.

Here, we demonstrate that swelling-induced mesoporous block copolymers can be templates for producing three-dimensional TiO2 networks by combining the atomic layer deposition technique.

We outline experiments on single-wall carbon nanotubes (SWNT) and exfoliated graphite (EG), as templates for producing nanostructures of the nanomagnetic manganese acetate cluster (Mn12[(CH2O 4O12]H2O)4O12] · 2CH3COOH · 4H2O),Mn12.

Gold nanoparticles were used by Singh and Lyon [ 57] as templates for producing hollow PNIAM beads.

The resulting plasmids were sequenced from both ends (performed at MicroSynth, Switzerland) and were used as templates for producing RNA probes for in situ hybridization (ISH).