Sentence examples for template for primary from inspiring English sources

In early development, airways serve as a template for primary blood vessel formation and, later, capillary beds guide alveolar formation.

The PCR reaction contained 1X Perkin-Elmer (Norwalk, CN) PCR buffer, 3 mM MgCl2, 200 µM (each) deoxynucleoside triphosphate, 200 nM of the forward and reverse primers, 5 units of Taq polymerase, and 0.5 2 µL of DNA template (for primary PCR) or 2 µL of primary PCR product (for secondary PCR) in a total 100-µL reaction mixture.

Repeats and all microscopy negative samples (due to an expected lower parasitaemia) were done under similar conditions with the exception that 2 µl DNA solution were used as template for the primary PCR.

Aliquots of 400 ng of total RNA were used for RT-PCR analysis (55°C for 35 min) in a 20 µl reaction using C. therm polymerase (Roche) a specific primer (4-1R; table S1). 3 µl of this reaction were used as template for a primary PCR performed by 20 cycles of 94°C (40 s), 60°C (40 s) and 72°C (80 s), with primers 4-1F and 4-1R (table S1).

All of the analyses will follow the template for the primary outcomes.

To generate unique sequences serial dilutions (1 50 and 1 500) of the ligation reaction were used as template for the primary PCR.

By providing a description of both the toolkit development and final product we hope to offer a template for other primary care practices interested in implementing panel management strategies.

The pellet was resuspended in normal saline (1 ml) and centrifuged at 12,000 rpm for 5 min. The supernatant was discarded and the pellet was resuspended in 200 μl of 0.1 × TE buffer before being boiled for 10 min and centrifuged at 12,000 rpm for 10 min. The supernatant was used as a template for the primary PCR.

Excised tissue was digested in 30 μl proteinase K (0.7 mg/ml; Boehringer Mannheim, Mannheim, Germany) at 50°C for 1 hour, which was then inactivated at 95°C for 10 min. This DNA preparation was used as a template for subsequent primary PCR reactions.

Design templates for five primary schools, five secondary schools and one all-through school have been created by 11 leading architectural practices, including Marks Barfield, the architects who gave us the London Eye; Alsop & Partners; Sarah Wigglesworth and Wilkinson Eyree.

The resulting tailed molecules were used as templates for a primary PCR with an anchor primer 5′ CTCTTGCTTGGATCCGGACCCCCCCCCCCCCDN 3′ plus a specific internal primer from close to the end of the known sequence.